Prophylactic Efficacy of a Combination of Proanthocyanidin and Vitamin E on Hepatotoxicity Induced by Doxorubicin in Rats

Doxorubicin (Dox) is a common chemotherapeutic anticancer drug. One of its side effect, according to some studies is oxidative stress-induced toxic changes on the liver. In this study protective efficacy of Proanthocyanidin (GSPE) along with vitamin E on Dox induced hepatotoxicity was evaluated in 50 male (divided in to five groups) Wister rats. After 2 weeks of the experiment and drug administration, blood samples and liver tissues were taken from all groups. Lipid profiles, liver function test in blood serum and Superoxide Dismutase (SOD), Catalase (CAT) and Malondialdehyde (MDA) levels in liver tissue were measured. We found that intraperitonial administration of Dox had profound effects on the liver as assessed by liver function tests and liver histology. The present results showed that pretreatment with proanthocyanidin extract, along with vitamin E enhances the antioxidant defense against Reactive Oxygen Species and reduced the oxidative stress and change in activity of antioxidant enzymes induced by Dox, thereby demonstrating the antioxidant potential of GSPE along with vitamin E in ameliorating the hepatotoxicity induced by Dox and it shows that it could be one approach to reduce the risk of Dox induced side effects in clinical settings.